The Optimization of Soluble PTEN Expression in Escherichia coli
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چکیده
منابع مشابه
The Optimization of Soluble PTEN Expression in Escherichia coli
As a vital tumor suppressor, PTEN (Phosphatase and tension homolog deleted on chromosome 10) is involved in inherited syndromes, and is among the most frequently inactivated tumor suppressor gene in sporadic cancers. PTEN loss-of-function widely occurs in human cancers via a variety of mechanisms, including genetic alterations and posttranslational modification. These suggest PTEN has a role of...
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reteplase is a segment of tissue plasminogen activator used for the removal of thrombi in blood vessels. in the present study the cloned reteplase gene was used for its expression in competent e. coli. the recombinant plasmid, pet15b/reteplase (rpet-bl21), was transformed into competent e.coli strain bl21 (de3) cells. overnight culture of the transformed bacteria was induced by the addition of...
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ژورنال
عنوان ژورنال: The Open Biochemistry Journal
سال: 2015
ISSN: 1874-091X
DOI: 10.2174/1874091x01509010042